Fig. 1: miR-5581-3p is a tumor suppressor in BCa.

a Relative contents of miR-5581-3p in BCa cells (T24 and UM-UC3) are in contrast with those in non-malignant urothelial cell lines (SV-HUC-1). b Comparison of the contents of miR-5581-3p in individual 20 pairs of BCa tissues are presented with the matching neighboring non-malignant tissues. c Kaplan–Meier survival data exhibits that upregulation of miR-5581-3p is remarkably related to a high OS rate of BCa. d CCK-8 data showed that the relative vitality of cells in the miR-5581-3p (50 nM)-inoculated groups of UM-UC3 and T24 cell lines was lower over time than the NC-inoculated groups. e Colony formation assays demonstrated that the rate of colony development was reduced in the miR-5581-3p (50 nM) mimic-inoculated groups compared to the NC-inoculated groups. f Cell cycle assays demonstrated over-expression of miR-5581-3p (50 nM) elevated the fraction of cells arrested in G1 phase in UM-UC3 and T24 cell lines. g Western blot data verified dampening of migration and proliferation-linked proteins via assessment of over-expression of miR-5581-3p (50 nM) in UM-UC3 and T24 cell lines. h Transwell assays revealed miR-5581-3p (50 nM) reduced the migration rate of UM-UC3 and T24 cell lines. i Wound-healing assays showed miR-5581-3p (50 nM) dampened the mobility of UM-UC3 cell line. Error bars designate SD acquired from three independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001.