Fig. 3: FTO silencing reduces cell migration and proliferation in BCa cell lines.
a CCK-8 assays demonstrated that the relative vitality of cells from siFTO (50 nM)-inoculated groups of UM-UC3 and T24 cell lines was lower over time than that of NC-inoculated groups. b Colony formation assays illustrated that the colony formation rate was lower for the siFTO (50 nM)-inoculated groups in contrast with that of the NC-inoculated groups. c Cell cycle assays demonstrated inhibition of FTO (50 nM) elevated the fraction of cells arrested in G1 phase in UM-UC3 and T24 cell lines. d Western blot data confirmed dampening of migration and proliferation-linked proteins were detected by the inhibition of FTO (50 nM) in UM-UC3, as well as T24 cell lines. e Transwell assays revealed siFTO (50 nM) reduced the migration rate of UM-UC3 and T24 cell lines. f Wound-healing assays showed siFTO (50 nM) inhibited the mobility of UM-UC3 cell line. g Kaplan–Meier survival data exhibits that upregulation of FTO is remarkably related to a low OS rate of BCa. h Dot-blot data indicated that the m6A content of UM-UC3 and T24 cell lines changed with the expression of miR-5581-3p and FTO. Error bars designate SD acquired from three independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001.
