Fig. 4: ORP5 overexpression suppresses lysosomes degradation of c-Met.
From: ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma
A Q-PCR analysis was carried out to examine c-Met mRNA levels after ORP5 overexpression and knockdown in RCC cells. B Western blot was carried out to assess the effects of overexpressing ORP5 on the half-life of c-Met in 786-O, ACHN cells treated with cycloheximide (CHX) at 20 µg/ml and collected at 0, 6, 12, and 24 h. C Western blot of c-Met expressions in the indicated cells treated with or without Chloroquine (100 µmol). D Immunoprecipitation and immunoblotting were performed to analyze the ubiquitination of c-Met when co-transfected with Ub and ORP5 expression plasmids in these two RCC cells. Error bars represent SD. *P < 0.05; **P < 0.01.
