Fig. 2: Knockdown of ANGPTL4 promotes the growth of MNNG cells in vitro. | Cell Death Discovery

Fig. 2: Knockdown of ANGPTL4 promotes the growth of MNNG cells in vitro.

From: ANGPTL4 negatively regulates the progression of osteosarcoma by remodeling branched-chain amino acid metabolism

Fig. 2

A–D Quantitation of ANGPTL4 mRNA and ANGPTL4 protein expression in MNNG-A4, MNNG-siA4, and control cell lines by RT-qPCR and western blot, respectively (N = 3, ****p < 0.0001 vs. the control group). E, G In vitro measurement of cell proliferation in two ANGPTL4-regulated MNNG cell lines (MNNG-A4, MNNG-siA4) and control cell lines. The cell index results indicate that cell proliferation is enhanced in the MNNG-siA4 cells but reduced in the MNNG-A4 cells compared to the control cells (N = 4). F, H Cell index at 48, 72, 96, and 120 h (N = 4, ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 vs. the control group). I, K Representative images of colony formation assays of the MNNG-A4, MNNG-siA4, and control cells. J, L Quantitation of colony counts after 2 weeks of in vitro maintenance (N = 3, *p < 0.05 vs. the control group).

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