Fig. 4: Low expression of ANGPTL4 promotes the accumulation of BCAAs and activates mTOR signaling. | Cell Death Discovery

Fig. 4: Low expression of ANGPTL4 promotes the accumulation of BCAAs and activates mTOR signaling.

From: ANGPTL4 negatively regulates the progression of osteosarcoma by remodeling branched-chain amino acid metabolism

Fig. 4

A, B Among the DEGs, genes that are highly related to BCAA metabolism were verified by RT-qPCR in the ANGPTL4-regulated cells and their controls (N = 3, ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 vs. the control group). C Proteins that are highly related to the BCAA metabolism were verified by western blot in the ANGPTL4-regulated cells and their controls. Quantitation of relative expression (N = 3, ns p > 0.05, *p < 0.05, **p < 0.01 vs. the control group). D The relative levels of BCAAs in MNNG-A4 cells, MNNG-siA4 cells, and their control cells (N = 2, *p < 0.05, vs. the control group). E Western blot detection of proteins in the mTOR signaling pathway showed that this pathway was activated in the MNNG-siA4 cells but inhibited in the MNNG-A4 cells.

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