Fig. 5: A BCAT inhibitor attenuates the elevated growth of MNNG-siA4 cells. | Cell Death Discovery

Fig. 5: A BCAT inhibitor attenuates the elevated growth of MNNG-siA4 cells.

From: ANGPTL4 negatively regulates the progression of osteosarcoma by remodeling branched-chain amino acid metabolism

Fig. 5

A In vitro measurement of cell attachment and cell proliferation in the MNNG-siA4 and control cell lines with 5 μM BCAT inhibitor treatment. B Cell index at 48, 72, 96, and 120 h with 5 μM BCAT inhibitor treatment. Mean ± SD (N = 4, ns p > 0.05, *p < 0.05, **p < 0.01 vs. the control group). C Representative images of colony formation assays of the MNNG-siA4 and control cells after 5 μM BCAT inhibitor treatment. D Quantitation of colony counts with 5 μM BCAT inhibitor treatment. Mean ± SD (N = 3, ns p > 0.05 vs. the control group). E, F Proteins that are highly related to the BCAA metabolism were verified by western blot in the MNNG-siA4 cells and the control cells after 5 μM BCAT inhibitor treatment (N = 3, ns p > 0.05, **p < 0.01, ***p < 0.001 vs. the control group). G The relative levels of BCAAs in the MNNG-siA4 cells and the control cells after 5 μM BCAT inhibitor treatment (N = 2, *p < 0.05 vs. the control group).

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