Fig. 6: A mTOR inhibitor abolishes the elevated growth of MNNG-siA4 cells. | Cell Death Discovery

Fig. 6: A mTOR inhibitor abolishes the elevated growth of MNNG-siA4 cells.

From: ANGPTL4 negatively regulates the progression of osteosarcoma by remodeling branched-chain amino acid metabolism

Fig. 6

A Western blot detection of proteins in the mTOR signaling pathway showed that activation of this pathway in MNNG-siA4 cells was reduced by mTOR inhibitor. B In vitro measurement of cell attachment and cell proliferation in the MNNG-siA4 and control cell lines with 0.2 μM mTOR inhibitor treatment. C Cell index at 24, 48, 72, and 96 h with 0.2 μM mTOR inhibitor treatment. Mean ± SD (N = 4, ns p > 0.05, *p < 0.05, vs. the control group). D Representative images of colony formation assays of the MNNG-siA4 and control cells after 0.2 μM mTOR inhibitor treatment. E Quantitation of colony counts with 0.2 μM mTOR inhibitor treatment. Mean ± SD (N = 3, **p < 0.01 vs. the control group).

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