Fig. 3: Panx1/ATP/P2RX7 axis is required for necrotic TECs-induced activation of NLRP3 inflammasome in macrophages. | Cell Death Discovery

Fig. 3: Panx1/ATP/P2RX7 axis is required for necrotic TECs-induced activation of NLRP3 inflammasome in macrophages.

From: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

Fig. 3

Representative immunofluorescence images of ATP in PMA-differentiated THP-1 cells (scale bar, 20 μm) (A). Quantification of ATP level in culture supernatant and in cell lysate of THP-1 cells by ATP assay kit (B, C). Relative mRNA expressions of Panx1, P2RX4, and P2RX7 in THP-1 cells (D). Representative and quantitative immunoblot analyses of Panx1 and P2RX7 (E, F). PMA-differentiated THP-1 cells were pretreated with probenecid (100 μM, 200 μM) or suramin (100 μM, and 200 μM) for 1 h and further stimulated with CM for 6 h. Representative and quantitative immunoblot analyses of the immature and mature forms of IL-1β in culture supernatants and cell lysates of THP-1 cells treated with probenecid or suramin (G, I). Quantification of IL-1β in supernatant of THP-1 cells treated with probenecid or suramin by ELISA (H, J). Data are presented as the mean ± SD of the representative experiment performed in at least three biological replicates. The p values were calculated using Students t test or one-way ANOVA test. *p < 0.05; **p < 0.01; ***p < 0.001.

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