Fig. 4: TLR2 is required for Panx1 cleavage and NLRP3 inflammasome activation in macrophages stimulated by necrotic TECs. | Cell Death Discovery

Fig. 4: TLR2 is required for Panx1 cleavage and NLRP3 inflammasome activation in macrophages stimulated by necrotic TECs.

From: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

Fig. 4

Relative mRNA expressions of TLR1, TLR2, TLR4, and TLR6 in THP-1 cells (A). Representative and quantitative immunoblot analysis of TLR2 in THP-1 cells (B, C). PMA-differentiated THP-1 cells were transfected with TLR2 siRNA for 36 h before incubated with CM. Representative and quantitative immunoblot analysis of the immature and mature forms of IL-1β in supernatant and cell lysates of THP-1 cells (D). Representative immunostaining images of ATP in THP-1 cells (scale bar, 30 μm) (E). Quantification of ATP in culture supernatant of THP-1 cells by ATP assay (F). Representative and quantitative immunoblot analyses of Panx1 and cleaved Panx1 in cell lysates of THP-1 cells (G). NC Negative control siRNA. Data are presented as the mean ± SD of the representative experiment performed in at least three biological replicates. The p values were calculated using Students t test or one-way ANOVA test. *p < 0.05; **p < 0.01; ***p < 0.001.

Back to article page