Fig. 1: GBM-EVs promote M2 polarization. | Cell Death Discovery

Fig. 1: GBM-EVs promote M2 polarization.

From: microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis

Fig. 1

A TEM results of GBM-EVs (scale bar = 100 nm). B NTA results of the size distribution of EVs. C Expression of marker proteins TSG101, CD63 and Calnexin in 5 μg GBM-EVs and 20 μg cell lysates determined using Western blot analysis. D Immunofluorescence images of EV uptake by macrophages under normoxia/hypoxia (double label; red represents EVs; green indicates macrophage) (scale bar = 25 μm). E RT-qPCR results of IL-10 and TNF-α mRNA expression in macrophages. F ELISA results of the secretion levels of IL-10 and TNF-α in the supernatant of macrophages. *p < 0.05 compared with the normal cells treated with PBS. # p < 0.05 compared with normal cells treated with GBM-EVs. The experiment was repeated 3 times independently.

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