Fig. 3: hsa-miR-27a-3p targets EZH1. | Cell Death Discovery

Fig. 3: hsa-miR-27a-3p targets EZH1.

From: microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis

Fig. 3

A Volcano plots displaying the differentially expressed genes retrieved from microarray dataset GSE12657. B Volcano plots displaying the differentially expressed genes retrieved from microarray dataset GSE104291. C Volcano plots displaying the differentially expressed genes retrieved from microarray dataset GSE50161. The X-axis represents -log10 (p value), the Y-axis represents logFoldChange, red dots represent upregulated genes, and green dots indicate downregulated genes. D Venn diagram displaying the intersection of target genes of hsa-miR-27a-3p predicted by bioinformatics analysis and differentially expressed genes retrieved from microarray datasets GSE12657, GSE104291 and GSE50161. E GO functional enrichment analysis on the intersected genes. X-axis represents gene ratio. Y-axis represents GO entries. The right histogram is color gradation. F Expression level of candidate genes in TCGA_LGG and GTEx. G The expression of EZH1 in microarray GSE50161. H The expression of EZH1 in clinical samples of patients with GBM (n = 50) and non-GBM patients (n = 20), *p < 0.05 compared with normal tissues. I The binding sites between hsa-miR-27a-3p and EZH1 3’UTR in human and mice predicted by starBase databse. J The targeting relationship between miR-27a and EZH1 verified by dual luciferase reporter gene assay, *p < 0.05 compared with EZH1 3’UTR-WT + mimic-NC group. The experiment was repeated 3 times independently.

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