Fig. 4: EZH1 suppresses M2 polarization by downregulating KDM3A. | Cell Death Discovery

Fig. 4: EZH1 suppresses M2 polarization by downregulating KDM3A.

From: microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis

Fig. 4

A ChIP-Seq data analysis results of the binding sites. B Enrichment of EZH1 and H3K27me3 on the KDM3A promoter in macrophages detected by ChIP analysis. C KDM3A mRNA expression in macrophages determined by RT-qPCR and H3K27me3 enrichment on the KDM3A promoter in macrophages detected by ChIP analysis. D mRNA expression of EZH1 in macrophages cultured under hypoxic conditions determined by RT-qPCR. E mRNA expression of KDM3A in macrophages cultured under hypoxic conditions determined by RT-qPCR. F mRNA expression of IL-10 and TNF-α in macrophages cultured under hypoxic conditions determined by RT-qPCR. G The expression of iNOS and Arg-1 detected by Western blot analysis. *p < 0.05 compared with IgG, si-NC or si-NC + sh-NC. # p < 0.05 compared with si-EZH1 + sh-NC. The experiment was repeated 3 times independently.

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