Fig. 5: KDM3A upregulates CTGF expression to boost M2 polarization. | Cell Death Discovery

Fig. 5: KDM3A upregulates CTGF expression to boost M2 polarization.

From: microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis

Fig. 5

A ChIP analysis results on enrichment of H3K4me1 and H3K27ac in the CTGF enhancer region of macrophages after addition of KDM3A. B ChIP analysis results on enrichment of H3K4me1 and H3K27ac in the CTGF enhancer region of macrophages after knockdown of KDM3A. C mRNA expression of KDM3A in macrophages cultured under hypoxic conditions determined by RT-qPCR. D mRNA expression of CTGF in macrophages cultured under hypoxic conditions determined by RT-qPCR. E mRNA expression of IL-10 and TNF-α in macrophages cultured under hypoxic conditions determined by RT-qPCR. F Western blot analysis results of the protein expression of iNOS and Arg-1. *p < 0.05 compared with IgG, si-NC or oe-NC + si-NC. #p < 0.05 compared with oe-KDM3A + si-NC. The experiment was repeated 3 times independently.

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