Fig. 6: GBM-EV-derived hsa-miR-27a-3p targets EZH1 to mediate M2 polarization through KDM3A/CTGF in vitro. | Cell Death Discovery

Fig. 6: GBM-EV-derived hsa-miR-27a-3p targets EZH1 to mediate M2 polarization through KDM3A/CTGF in vitro.

From: microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis

Fig. 6

A mRNA expression of EZH1 in macrophages after EZH1 and CTGF knockdown determined using RT-qPCR. B mRNA expression of KDM3A in macrophages after EZH1 and CTGF knockdown determined using RT-qPCR. C mRNA expression of CTGF in macrophages after EZH1 and CTGF knockdown determined using RT-qPCR. D mRNA expression of IL-10 and TNF-α in macrophages after EZH1 and CTGF knockdown determined using RT-qPCR. E mRNA expression of EZH1 in macrophages overexpressing CTGF evaluated using RT-qPCR. F mRNA expression of KDM3A in macrophages overexpressing CTGF evaluated using RT-qPCR. G mRNA expression of CTGF in macrophages overexpressing CTGF evaluated using RT-qPCR. H Expression of hsa-miR-27a-3p after macrophages were transfected with hsa-miR-27a-3p mimic/inhibitor determined using RT-qPCR. I Protein expression of EZH1, KDM3A and CTGF in macrophages measured using Western blot analysis. J mRNA expression of IL-10 and TNF-α in macrophages detected using RT-qPCR. K Protein expression of iNOS and Arg-1 in macrophages determined using Western blot analysis. L CCK-8 assay results of proliferation ability of GBM cells. M Transwell assay results of the migration and invasion ability of GBM cells (scale bar = 50 μm). *p < 0.05 compared with si-NC + sh-NC, oe-NC or oe-CTGF + EVs-mimic-NC. #p < 0.05 compared with si-EZH1 + sh-NC or oe-CTGF + EVs-inhibitor-NC. The experiment was repeated 3 times independently.

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