Fig. 7: GBM-EV-derived hsa-miR-27a-3p aggravates GBM in vivo. | Cell Death Discovery

Fig. 7: GBM-EV-derived hsa-miR-27a-3p aggravates GBM in vivo.

From: microRNA-27a-3p delivered by extracellular vesicles from glioblastoma cells induces M2 macrophage polarization via the EZH1/KDM3A/CTGF axis

Fig. 7

Nude mice were injected with EVs from GBM cells overexpressing hsa-miR-27a-3p or/and macrophages transfected with lentivirus-mediated CTGF. A hsa-miR-27a-3p expression and CTGF mRNA expression in each group of mice (n = 6) determined using RT-qPCR. B H&E staining results of GBM cell invasion to the mouse brain tissues (n = 6) (scale bar = 50 μm). C Statistical data of survival time of mice in each group (n = 6). D Western blot analysis results of the protein expression of EZH1, KDM3A and CTGF in mouse brain tissues of each group (n = 6). E ELISA results of secretion levels of IL-10 and TNF-α in peripheral serum of mice (n = 6). F ELISA results of content levels of IL-10 and TNF-α in lysate of mouse tumor xenografts (n = 6). *p < 0.05 compared with sham-operated mice. #p < 0.05 compared with mice harboring EVs containing mimic-NC. & p < 0.05 compared with mice harboring EVs containing hsa-miR-27a-3p mimic + sh-NC.

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