Fig. 2: Characteristics of circDHTKD1 in BCa cells.
From: circDHTKD1 promotes lymphatic metastasis of bladder cancer by upregulating CXCL5
A Schematic diagram showing the circularization of circDHTKD1 derived from exons 2 to 16 of DHTKD1. The PCR product of circDHTKD1 was verified by Sanger sequencing. Red arrow indicates the back-splice junction site of circDHTKD1. B PCR for circDHTKD1 and DHTKD1 in cDNA and gDNA of 5637 and T24. GAPDH was applied as negative control. C qRT-PCR was used to detect the expression of circDHTKD1 and DHTKD1 mRNA in 5637 and T24 after treatment with RNase R. D and E RNA fractionation and FISH assay in T24 cells to detect the location of circDHTKD1. Scale bars, 20 µm. F qRT-PCR was used to detect the abundance of circDHTKD1 and DHTKD1 mRNA in T24 cells after treatment with Actinomycin D. Error bars indicate standard deviations of three independent experiments. *p < 0.05 and **p < 0.01.
