Fig. 1: EZH2 silencing inhibits the proliferation and induces the senescence of GIST cells.
From: SMYD2 aggravates gastrointestinal stromal tumor via upregulation of EZH2 and downregulation of TET1
A RT-qPCR detection of EZH2 expression in cancer and adjacent normal tissues from 46 GIST patients. B RT-qPCR detection of EZH2 expression in GIST-T1 cells treated with sh-EZH2-1, sh-EZH2-2, or sh-EZH2-3. C Western blot analysis of the expression of EZH2 in GIST-T1 cells treated with sh-EZH2-1, sh-EZH2-2, or sh-EZH2-3. D CCK-8 detection of GIST-T1 cell viability after silencing EZH2. E GIST-T1 cell colony formation ability after silencing EZH2 evaluated by soft agar colony formation assay. F BrdU/PI cell cycle analysis of GIST-T1 cell cycle distribution after silencing EZH2. G SA-β-gal detection of ratio of senescent GIST-T1 cells after silencing EZH2 (1 mL SA-β-gal is used per experiment). *p < 0.05 vs. the adjacent normal tissues or sh-NC-treated GIST-T1 cells. Measurement data were expressed as mean ± standard deviation. Comparison between cancer tissues and adjacent normal tissues was conducted using paired t-test, while comparison between the other two groups was conducted by unpaired t-test. The cell experiment was repeated three times.
