Fig. 4: TET1 silencing abolishes the effect of EZH2 silencing on GIST cells.
From: SMYD2 aggravates gastrointestinal stromal tumor via upregulation of EZH2 and downregulation of TET1
GIST-T1 cells were treated with sh-NC, sh-EZH2, sh-TET1, or sh-EZH2 + sh-TET1. A Western blot analysis of TET1 and p53 protein expression in GIST-T1 cells. B BrdU/PI cell cycle assay to quantify GIST-T1 cell cycle distribution. C SA-β-gal detection of ratio of senescent GIST-T1 cells. D Western blot analysis of cyclin A protein expression in GIST-T1 cells. *p < 0.05 vs. sh-NC-treated GIST-T1 cells, #p < 0.05 vs. sh-EZH2-treated GIST-T1 cells. Measurement data were expressed as mean ± standard deviation. Unpaired t-test was used for two-group data comparison. One-way ANOVA with Tukey’s post-hoc test was used to compare multigroup data. The cell experiment was repeated three times.
