Fig. 2: TET2 knockout affects early skeletal muscle development by inhibiting MuSC proliferation and differentiation.

A Immunofluorescence staining of Laminin of TA muscles from 1-week-old WT and TET2 KO mice (Scale bars, 50 μm) B Quantification of the number of myofibers and cross-section area of TA muscles isolated from 1-week-old WT and TET2 KO mice (n = 3 biological samples) C Immunofluorescence staining of PAX7 and MCM2 of muscle tissue from neonatal WT and TET2 KO mice (Scale bars, 25 μm) D Quantification of the number of PAX7⁺ cell per mm² and the percentage of PAX7⁺ MCM2⁺ cell in neonatal WT and TET2 KO mice (n = 6 biological samples) E Fluorescence staining of EdU in fresh-isolated MuSCs from WT or TET2 KO mice (Scale bars, 50 µm). F Quantification of the percentage of EdU⁺ MuSCs (n = 3 biological samples) and the relative expression levels of the proliferation related genes in WT and TET2-KO MuSCs. (n = 3 biological samples) G Immunofluorescence staining of MHC in differentiated myotubes from WT and TET2-KO MuSCs (Scale bars, 50 µm). H Quantification of the fusion index in differentiated myotubes (n = 3 biological samples) and relative expression levels of the myogenic differentiation related genes in WT and TET2-KO MuSCs (n = 3 biological samples). Error Bar indicated SEM. ns indicated that Not Significant, * indicated that p < 0.05, ** indicated that p < 0.01, *** indicated that p < 0.001.