Fig. 3: TET2 knockout mice exhibit delayed muscle repair and regeneration due to the impaired MuSC proliferation.
From: Disturbance of calcium homeostasis and myogenesis caused by TET2 deletion in muscle stem cells
A Schematic diagram of the muscle regeneration experiment. 100 μl 10 μM CTX was injected into TA tissue of 8-week-old WT and TET2-KO mice. B Immunofluorescence staining of laminin of TA muscles after injury (Scale bars, 50 μm). C Immunofluorescence staining of eMHC of TA muscles after injury (Scale bars, 50 μm) D Immunofluorescence staining of PAX7 and EdU at 3 dpa (Scale bars, 50 μm) and quantification of the percentage of PAX7+ EdU+ cell (n = 3 biological samples). E Immunofluorescence staining of PAX7 and EdU at 20 dpa (Scale bars, 50 μm) and quantification of the percentage of PAX7+ EdU+ cell (n = 3 biological samples). Error Bar indicated SEM. * indicated that p < 0.05, ** indicated that p < 0.01.
