Fig. 6: Slc8a3 partially rescues the myogenic capacity of TET2-KO MuSCs.
From: Disturbance of calcium homeostasis and myogenesis caused by TET2 deletion in muscle stem cells
A The Fluo-3 probe of Ctrl, TET2-KO and TET2-KO-Slc8a3 group (Scale bars, 50 µm). B Quantification of the intensity of Fluo-3 of Ctrl, TET2-KO, and TET2-KO-Slc8a3 group (n = 3 biological samples). C Relative expression levels of Ca-signaling related genes in Ctrl, TET2-KO and TET2-KO-Slc8a3 group (n = 3 biological samples). D Immunofluorescence staining of EdU in Ctrl, TET2-KO, and TET2-KO-Slc8a3 group (Scale bars, 50 µm). E Quantification of the percentage of EdU+ in Ctrl, TET2-KO, and TET2-KO-Slc8a3 group (n = 3 biological samples). F Relative expression levels of the proliferation related genes in Ctrl, TET2-KO, and TET2-KO-Slc8a3 group (n = 3 biological samples). G Immunofluorescence staining of MHC in differentiated myotubes from Ctrl, TET2-KO, and TET2-KO-Slc8a3 group (n = 3 biological samples). H Quantification on the fusion index of differentiated myotubes from Ctrl, TET2-KO, and TET2-KO-Slc8a3 group (n = 3 biological samples). I Relative expression levels of the myogenic related genes in Ctrl, TET2-KO, and TET2-KO-Slc8a3 group (n = 3 biological samples). Error Bar indicated sem ns indicated that Not Significant, * indicated that p < 0.05, ** indicated that p < 0.01, *** indicated that p < 0.001.
