Fig. 2: CircEIF3H was essential for TNBC progression.

A Schematic illustration of siRNAs specific to the back-splicing junction of circEIF3H and the interfering efficacies measured by qRT-PCR. B–D The effects of circEIF3H knockdown on the proliferation of MDA-MB-231 and MDA-MB-468 cells were examined by MTT (B), EdU (C), and colony formation assays (D). E Cell cycle distributions in circEIF3H knockdown cells were presented by flow cytometry. F Transwell migration and invasion assays were used to evaluate the motility of MDA-MB-231 and MDA-MB-468 cells transfected with si-NC or si-circEIF3H. The quantitative data were presented as mean ± S.D. Statistical significance was determined by two-sided Student’s t-test, **P < 0.01, and ***P < 0.001. Cell experiments were repeated three times.