Fig. 2: TS induced apoptosis and blocked cell cycle of hepatoma cells in vitro.

The effect of TS with 15 μmol/L on apoptotic rate of HepG2 (A) and Huh7 (B) after 72 h using annexin V/PI double staining. C The effects of TS with 15 μmol/L on the cell cycle of HepG2 through propidium iodide staining. D The expression of Cyclin D1 after treated with TS by WB assay and the statistical analysis. Results were obtained from experiments carried out in triplicate from at least three independent experiments. Data are presented as the mean ± SD (n = 5). *p < 0.05 vs. the Control group; **p < 0.01 vs. the Control group; ***p < 0.001 vs. the Control group.