Fig. 4: TS promoted the immune regulation of H22 tumor bearing mice.

A The proportion of CD4+ T cells in the spleen of tumor bearing mice were detected by flow cytometry. B The analysis of the results from A. C The proportion of CD8+ T cells in the spleen of tumor bearing mice were detected by flow cytometry. D The analysis of the results from C. The infiltration of CD4+ (E) /CD8+ (F) T cells in tumor tissue sections were detected by immunofluorescence (20×). Results are from independent duplicate or triplicate experiments and presented as means ± SD. Number of mice per group are seven (n = 7). *p < 0.05 vs. the Control group; **p < 0.01 vs. the Control group; ***p < 0.001 vs. the Control group.