Fig. 4: MiR-494-3p is a molecular sponge for lncRNA SNHG12. | Cell Death Discovery

Fig. 4: MiR-494-3p is a molecular sponge for lncRNA SNHG12.

From: Identifying a novel KLF2/lncRNA SNHG12/miR-494-3p/RAD23B axis in Spare Nerve Injury-induced neuropathic pain

Fig. 4

Biotinylated RNA pull-down assay followed by qRT-PCR assay was performed to assess relative expression levels of putative miRNA targets of lncRNA SNHG12 in PC12 cells (A) and 293 T cells (B). C The relative expression level of miR-494-3p in lncRNA SNHG12 downregulated PC12 cells was measured by qRT-PCR. D Predicted binding sequences between lncRNA SNHG12 and miR-494-3p are shown. The binding possibility between lncRNA SNHG12 and miR-494-3p was verified by a luciferase reporter gene assay in PC12 cell (E) and 293 T cell (F). G The relative expression level of miR-494-3p in the DRGs of rats from the Sham or SNI groups was measured by qRT-PCR. Experimental data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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