Fig. 6: MiR-494-3p directly targets RAD23B. | Cell Death Discovery

Fig. 6: MiR-494-3p directly targets RAD23B.

From: Identifying a novel KLF2/lncRNA SNHG12/miR-494-3p/RAD23B axis in Spare Nerve Injury-induced neuropathic pain

Fig. 6

A The intersection results of the bioinformatics analysis of miR-494-3p potential targets are illustrated in a scheme. B Relative expression levels of putative mRNA targets in miR-494-3p overexpressed PC12 cells were measured by qRT-PCR. C Predicted binding sequences between RAD23B and miR-494-3p are shown. The binding possibility between RAD23B and miR-494-3p was verified by a luciferase reporter gene assay in microglial cells (D) and 293 T cells (E). The relative expression of RAD23B in the DRGs isolated from SNI or Sham rats at the indicated time points was assessed by qRT-PCR (F) and western blot (G). Experimental data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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