Fig. 2: CB1 activation suppressed the proliferation, migration and invasion of colorectal cancer cells. | Cell Death Discovery

Fig. 2: CB1 activation suppressed the proliferation, migration and invasion of colorectal cancer cells.

From: Cannabinoid Receptor-1 suppresses M2 macrophage polarization in colorectal cancer by downregulating EGFR

Fig. 2

A Western blot assay tested the protein level of CB1 after ACEA and AM251 treatment. B Western blot analysis showed that CB1 activation significantly decreased the expression of EGFR while its inhibition increased the expression of EGFR. C, D Colony forming assay showed that CB1 activation suppressed colony formation of colorectal cancer cells, while its inhibition promoted colony formation. E, F Wound healing test showed that CB1 activation prevented cell migration while CB1 inhibition enhanced cell migration of colorectal cancer cells. G, H Transwell invasion assay demonstrated that CB1 activation blocked cell invasion while CB1 inhibition enhanced cell invasion of colorectal cancer cells. *P < 0.05; **P < 0.01; ***P < 0.001.

Back to article page