Fig. 2: Relationship between methylation level of PAX8 and TP53 mutation (CNH) in molecular subtyping of UCEC.

A The methylation levels of PAX8 (pair3) in HEC-1B, Ishikawa, 12Z, and HeLa cells were detected by bisulfite conversion sequencing. B Schematic diagram of methylation information of all sites in pair3. C Statistical table of methylation information for all sites in pair3. D Promoter methylation level of PAX8 in UCEC was analyzed by TCGA samples. E Promoter methylation level of PAX8 in UCEC based on TP53 muation status was analyzed by TCGA samples. The Beta value indicates level of DNA methylation ranging from 0 (unmethylated) to 1 (fully methylated). F Expression of PAX8 in UCEC based on TP53 muation status was analyzed by TCGA samples. G Protein level of PAX8 in UCEC based on p53-related pathway. H The proportion of PAX8 copy amplification/mRNA upregulation (Altered group) in four molecular subtypes of endometrial cancer. *p < 0.01, **p < 0.005, ***p < 0.001.