Fig. 4: C-MYC gene activation is responsible for PAX8 overexpression in UCEC. | Cell Death Discovery

Fig. 4: C-MYC gene activation is responsible for PAX8 overexpression in UCEC.

From: Paired box 8 facilitates the c-MYC related cell cycle progress in TP53-mutation uterine corpus endometrial carcinoma through interaction with DDX5

Fig. 4

A Representative enrichment plots of the c-MYC-related gene set which negatively correlate with siPAX8 in Ishikawa cell. B PAX8 expression was knocked down by siRNA transfection, cells were collected 48 h after transfection, the expression level of PAX8 and c-MYC proteins in Ishikawa and HEC-1B cells were detected by Western blotting. C After gradient knockdown of PAX8 in Ishikawa cells, the expression level of PAX8 and cell cycle-related proteins were detected by Western blotting. D, E PAX8 was overexpressed by transfected HA-PAX8 plasmid (0ug, 0.5ug, 1ug, 1.5ug, 2ug) in 12Z cells, after 48 h transfection, total RNA and total protein were extracted from cells for qPCR and Western blot analysis, respectively. F Linear regression and correlation of PAX8 versus c-MYC protein level. R value is Pearson’s correlation coefficient. G The mRNA expression level of c-MYC was positively correlated with PAX8 in GEPIA. N.S. not significantly different among the groups by Student’s t-test. *p < 0.01, **p < 0.005, ***p < 0.001.

Back to article page