Fig. 5: DDX5 is a partner of PAX8. | Cell Death Discovery

Fig. 5: DDX5 is a partner of PAX8.

From: Paired box 8 facilitates the c-MYC related cell cycle progress in TP53-mutation uterine corpus endometrial carcinoma through interaction with DDX5

Fig. 5

A PAX8-interacting proteins in Ishikawa cell were precipitated by using anti-PAX8 antibody, and identified by LC-MS/MS analyses. The proteins identified only in PAX8 precipitates are listed. B Co-IP analyses of HA-PAX8 and DDX5-GFP interaction in HEK293 cells. C Western analyses of the nuclear and cytoplasmic fractionated samples from 12Z and Ishikawa cells. Lamin B and tubulin were used as the nuclear (N) and cytoplasmic (C) markers, respectively. D Immunofluorescence was used to determine the localization of PAX8 and DDX5 in 12Z and Ishikawa cells. DAPI staining was included to visualize the cell nucleus (Blue). Scale bar = 200 μm in 100x and 100μm in 200x vision. E PAX8 was knocked down in Ishikawa cells by using siRNA, after 48 h transfection, total protein was extracted from cells for Western blot analysis. F Gradient transfection of DDX5 plasmid in 12z cells, 24 h later, RNA was extracted to detect the expression of DDX5, c-MYC, and PAX8, Hprt was used as a housekeeping gene.

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