Fig. 6: PAX8 promotes UCEC cell growth and cell cycle progress dependent on DDX5 and c-MYC in vitro. | Cell Death Discovery

Fig. 6: PAX8 promotes UCEC cell growth and cell cycle progress dependent on DDX5 and c-MYC in vitro.

From: Paired box 8 facilitates the c-MYC related cell cycle progress in TP53-mutation uterine corpus endometrial carcinoma through interaction with DDX5

Fig. 6

A EdU incorporation assays of HEC-1b cell transiently transfected with siNC or siPAX8 or c-MYC or siPAX8 + c-MYC. B The percentage of EdU positive cells was blindly calculated with counting nine nonoverlaping fields. Values are means ± s.d. C HEC-1B cells were transfected with siRNAs or plasmid to knockdown PAX8 or overexpression c-MYC and DDX5, respectively. The proteins were extracted from cells for Western blotting analysis. D, E HEC-1B cells were transfected with plasmid or siRNA to knockdown PAX8 and overexpression c-MYC and DDX5, after treatment for 48 h. Cells were assayed by flow cytometry. The percentages of the cells in G1, S, and G2 phase were counted and compared. F HEC-1b cells were transfected with siPAX8 or siPAX8 + c-MYC or siPAX8 + DDX5. Representative G1/S transition markers were immunoblotted.

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