Fig. 1: EndMT existed in both in vitro and in vivo KD models. | Cell Death Discovery

Fig. 1: EndMT existed in both in vitro and in vivo KD models.

From: CircRNA-3302 promotes endothelial-to-mesenchymal transition via sponging miR-135b-5p to enhance KIT expression in Kawasaki disease

Fig. 1

a–c HUVECs were treated with medium containing 15% sera from health control (HC) (n = 6) or KD subjects (KD) (n = 6). After 48 h, the treated endothelial cells were harvested. ZO-1 and Vimentin mRNA levels were measured by RT-qPCR analysis (a). The protein levels of Twist, Snail, Vimentin and ZO-1 were examined by western blotting (b). The expression of Vimentin and ZO-1 were analyzed by immunofluorescence staining (c). Magnification: ×400 for Vimentin staining, and magnification:×1000 for ZO-1 staining. Scale bar = 50 μm for c. d Migration ability was measured by transwell test. Magnification: ×40. Scale bar = 100 μm. e After the mice were injected with PBS or CAWS, they were fed normally for 6 weeks, and the heart aorta root sections were obtained for HE staining. Magnification: ×40. Scale bar = 100 μm. f–g The heart tissues were harvested, and the total RNA and protein were respectively isolated for determining the expression of endothelial and mesenchymal markers. h Vimentin expression was analyzed in coronary endothelial cells using Vimentin/CD31 double staining. Magnification:×400. Scale bar = 50 μm. *P < 0.05 and **P < 0.01 vs. the HC group or PBS group.

Back to article page