Fig. 3: Upregulation of circ3302 induced EndMT. | Cell Death Discovery

Fig. 3: Upregulation of circ3302 induced EndMT.

From: CircRNA-3302 promotes endothelial-to-mesenchymal transition via sponging miR-135b-5p to enhance KIT expression in Kawasaki disease

Fig. 3

a HUVECs were treated with medium containing 15% sera from HC (n = 6) and KD subjects (n = 6). After 48 h, circ3302 levels were measured by RT-qPCR analysis. b HUVECs were transfected with Plasmid-circ3302 (P-circ3302) to overexpress circ3302. c, d The mRNA levels of Vimentin and ZO-1 were determined by RT-qPCR analysis. e The protein levels of Twist, β-catenin, Vimentin, Claudin-1 and ZO-1 were determined by Western blotting. f, g Effect of circ3302 overexpression on migration was respectively evaluated by scratch and transwell assays. Magnification:×40 and scale bar = 100 μm for scratch assay. Magnification:×100 and scale bar = 50 μm for transwell assay. Data were expressed as mean ± SD (n = 3). *P < 0.05 vs. the HC group or NC group.

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