Fig. 4: Effect of circ3302 silencing on KD serum-induced EndoMT. | Cell Death Discovery

Fig. 4: Effect of circ3302 silencing on KD serum-induced EndoMT.

From: CircRNA-3302 promotes endothelial-to-mesenchymal transition via sponging miR-135b-5p to enhance KIT expression in Kawasaki disease

Fig. 4

a–c HUVECs were transfected with si-circ3302, and then treated with medium containing 15% sera from HC (n = 6) and KD subjects (n = 6) for 48 h. Next, the expression of circ3302, Vimentin and ZO-1 was measured by RT-qPCR analysis. d Endothelial cells were treated as above, and then the protein levels of ZO-1, Claudin-1, Twist, Vimentin, and β-catenin were measured by western blot analysis. e, f Effect of circ3302 silencing on KD serum-induced migration was evaluated using scratch and transwell assays. Magnification:×40 and scale bar = 100 μm for scratch assay. Magnification:×100 and scale bar = 50 μm for transwell assay. *P < 0.05 vs. the HC group or KD group.

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