Fig. 5: Changes of gene expression after circ3302 overexpression in HUVECs and the relationship between circ3302 and KIT. | Cell Death Discovery

Fig. 5: Changes of gene expression after circ3302 overexpression in HUVECs and the relationship between circ3302 and KIT.

From: CircRNA-3302 promotes endothelial-to-mesenchymal transition via sponging miR-135b-5p to enhance KIT expression in Kawasaki disease

Fig. 5

a Heatmap illustrated the gene expression profiling of HUVECs after circ3302 overexpression. b Gene expression profiling (Volcano plot) of HUVECs after circ3302 was overexpressed. c KEGG pathway analysis was conducted in HUVECs with circ3302 overexpression. d HUVECs were transfected with plasmid-circ3302, and the mRNAs levels of FAM156A, HIST1H4K, KIT, NR4A3, RELN, and RGS7BP were detected by RT-qPCR analysis. e–g After transfected with plasmid-circ3302 or plasmid-circ3302 plus si-circ3302, the expression of KIT was determined at mRNA levels and protein levels. Significance: *P < 0.05, and **P < 0.01 vs. the NC group or Plasmid-circ3302 group. Plasmid+si-circ3302, indicated plasmid-circ3302 plus si-circ3302.

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