Fig. 6: Knowing down of KIT alleviates KD serum-mediated EndMT process. | Cell Death Discovery

Fig. 6: Knowing down of KIT alleviates KD serum-mediated EndMT process.

From: CircRNA-3302 promotes endothelial-to-mesenchymal transition via sponging miR-135b-5p to enhance KIT expression in Kawasaki disease

Fig. 6

a HUVECs were transfected with si-KIT, and then treated with medium containing 15% sera from HC (n = 6) and KD subjects (n = 6) for 48 h. After that, the mRNA levels of Vimentin, α-SMA, ZO-1, and VE-cadherin were examined by RT-qPCR analysis. b The protein levels of ZO-1, Claudin-1, Twist, and Vimentin were determined by Western blotting. c Migration ability was analyzed by scratch assay (n = 3) Magnification:×40 and scale bar = 100 μm for scratch assay. Data were shown as mean ± SD, and *P < 0.05 vs. the HC group or KD group.

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