Fig. 7: Circ3302 bound miR-135b-5p to regulate KIT expression. | Cell Death Discovery

Fig. 7: Circ3302 bound miR-135b-5p to regulate KIT expression.

From: CircRNA-3302 promotes endothelial-to-mesenchymal transition via sponging miR-135b-5p to enhance KIT expression in Kawasaki disease

Fig. 7

a TargetScan, miRanda and RNAhybrid were used to predict the potential miRNAs that bind to circ3302. TargetScan and RNAhybrid were utilized to screen potential miRNAs that target KIT. Then miRNAs that both bind to circ3302 and target KIT were further obtained via Venn diagram. b BiBiServ2 and TargetScan were used to predict the binding capacity and scores of circRNAs/KIT and miRNAs. c The predicted binding sites between circ3302 and miR-135b-5p, and between miR-135b-5p and KIT. d, e Luciferase results between circ3302 and miR-135b-5p, and between KIT and miR-135b-5p. Data were presented as mean ± SD (n = 3), and *P < 0.05 vs. the blank group. f, g The protein and mRNA levels of KIT were respectively examined in HC group, KD group, and KD + Si-circ3302 group. Data were shown as mean ± SD (n = 3), and *P < 0.05 and **P < 0.01 vs. the HC group or KD group. h, i The protein and mRNA expression of KIT was examined in KD group, KD + mimic group, KD + Si-circ3302 group, and KD + Si-circ3302 + mimic group. j Distribution of circ3302 was analyzed in the nucleus and cytoplasm. Data were expressed as mean ± SD (n = 3), and *P < 0.05 vs. the HC Nuclear group or KD Nuclear group. k Schematic diagram about the mechanism of circ3302 inducing EndMT process. KD serum increased the expression of circ3302, which then bound to miR-135b-5p to inhibit its binding with KIT mRNA, finally resulting in elevated KIT translation and subsequent EndMT in KD.

Back to article page