Fig. 6: Gankyrin promotes glycolysis through YAP1. | Cell Death Discovery

Fig. 6: Gankyrin promotes glycolysis through YAP1.

From: Gankyrin modulated non-small cell lung cancer progression via glycolysis metabolism in a YAP1-dependent manner

Fig. 6

Relative ATP content (A, D), glucose consumption (B, E), and lactic acid production (C, F) were determined in A549 and H460 cells transfected with YAP1-overexpression plasmid or with Gankyrin-overexpression plasmid and YAP1 siRNA or si-NC (n = 4, *P < 0.05, **P < 0.01). G ECAR was measured using a Seahorse Bioscience Extracellular Flux Analyzer after transfection with Gankyrin and si-YAP1 in A549 cells. ECAR curves were obtained after treatment with glucose, oligomycin, and 2-DG. Black arrows represent the time of cell treatment (n = 3, *P < 0.05). H OCR was measured using Seahorse Bioscience Extracellular Flux Analyzer after transfecting A549 cells with Gankyrin and si-YAP1. OCR curves were obtained after treatment with oligomycin, FCCP, and rotenone/antimycin A. Black arrows indicate the time of treatment (n = 3, ns means no significant).

Back to article page