Fig. 2: Helicobacter pylori infection downregulates DLC1 expression in vivo.
From: Functional antagonism between CagA and DLC1 in gastric cancer
A DLC1 positivity in uninfected and H. pylori-infected human gastric biopsies. Sections from FFPE human stomach tissue (n = 9 Hp+ vs. n = 10 Hp- cases) from endoscopic material were stained with DLC1 Ab by IHC. Positivity scores for DLC1 were correlated to the infection status. Data are absolute case numbers after dichotomous grouping of stainings as negative (scores 0/1) vs. positive (scores 2/3) (n.s., Hp+ vs. Hp-, Fisher-Exact test). B H. pylori-induced inflammatory cell infiltrates outcompete DLC1+ cells in human gastric biopsies. Representative IHC images from (A) of the corpus region (left, healthy; right, gastritis). Colour code: Brown = DLC1; Blue = nuclei (hematoxylin). Original magnifications ×200; Scale bar = 50 µm. Note the displacement of DLC1+ cells by accumulating intramucosal leucocytes in inflammatory regions. C H. pylori downregulates DLC1+ positivity in vivo. C57BL/6J mice were infected with mouse-adapted CagA+ injection-proficient H. pylori PMSS1 WT strain or CagA-injection-deficient isogenic mutant ΔCagE for 1 or 6 months. Sections from FFPE gastric tissue were stained with Abs against chief/stem cell marker MIST1 or DLC1. Quantitative analyses (top) and representative IHC images of the corpus region (bottom). Data are cell numbers per mm2 (*p < 0.05 vs. WT, Kruskal–Wallis test with Dunn or Sidak post-tests, n = 2–9 mice per group or time point). Colour code: Brown = DLC1/MIST1; Blue = nuclei (hematoxylin). Original magnifications ×100; Scale bar = 100 µm.
