Fig. 2: POLRMT silencing or KO inhibits skin SCC cell proliferation and migration.
From: The mitochondrial RNA polymerase POLRMT promotes skin squamous cell carcinoma cell growth
Stable C1 primary human skin SCC cells, expressing the lentiviral POLRMT shRNA (“shPOLRMT”), the CRISPR/Cas9-POLRMT-KO construct (“koPOLRMT”) or scramble control shRNA plus Cas9 control construct (“c-sh+Cas9”), were established; Expression of listed genes and proteins was tested (A–C); Cells were further cultured for the designated time periods, cell viability, proliferation and migration were tested by CCK-8 (D), nuclear EdU staining (E) and “Transwell” (F) assays, respectively. “Pare” stands for parental control C1 primary cells. *P < 0.05 versus “Pare” cells. Scale bar = 100 μm (E, F).
