Fig. 6: POLRMT shRNA virus injection hinders subcutaneous A431 xenograft growth.
From: The mitochondrial RNA polymerase POLRMT promotes skin squamous cell carcinoma cell growth
The nude mice bearing the subcutaneous A431 xenografts were subject to intratumoral injection of the POLRMT-shRNA-expressing AAV (“AAV-shPOLRMT”) or scramble control shRNA AAV (“AAV-c-sh”). Virus was injected daily for four consecutive days. Tumor volumes (A) and mice body weights (D) were recorded weekly for six consecutive weeks (“Day-0” to “Day-42”). The estimated daily tumor growth, in mm3 per day, was calculated as described (B). All experimental animals were sacrificed by decapitation at “Day-42”, and xenograft tumors were carefully isolated and weighed individually (C). Expression of listed genes and proteins in the tumor lysates of the described tumors was tested by qRT-PCR (E and G) and Western blotting (F and H) assays. TBAR activity (I) and ATP contents (J) were tested as well. Data were mean ± standard deviation (SD). *P < 0.05 versus “AAV-c-sh” group.
