Fig. 4: METTL3 inhibited lipolysis by downregulating MGLL expression.

A The mRNA levels of MGLL were increased by METTL3 downregulation and decreased by METTL3 overexpression. B MeRIP-qPCR showed that the levels of m6A modification on MGLL mRNA were decreased by METTL3 downregulation and increased by METTL3 overexpression. C, D The mRNA and protein levels of MGLL in adipocytes transfected with siRNAs. E, F METTL3 downregulation increased the levels of FFA release and glycerol release and downregulation of MGLL rescued these effects; n = 9, *P < 0.05, **P < 0.01, ns indicates not significant. Control means treatment of negative control siRNA in A (left), B (left), C-F and means treatment of negative control lentivirus in A (right), B (right).