Fig. 5: METTL3 accelerated the degradation of MGLL mRNA via YTHDF2. | Cell Death Discovery

Fig. 5: METTL3 accelerated the degradation of MGLL mRNA via YTHDF2.

From: Intermittent hypoxia-induced METTL3 downregulation facilitates MGLL-mediated lipolysis of adipocytes in OSAS

Fig. 5

A METTL3 downregulation increased the half-life of MGLL mRNA. B METTL3 overexpression reduced the half-life of MGLL mRNA. C, D The mRNA and protein levels of YTHDF2 in adipocytes transfected with siRNAs. E, F The mRNA and protein levels of MGLL in adipocytes transfected with siRNAs targeting for YTHDF2, YTHDC2 or YTHDF3. G RNA pulldown showed that the YTHDF2 protein was interacted with MGLL mRNA but not antisense RNA. H RIP assay showed that MGLL mRNA was precipitated by YTHDF2 protein but not control IgG; n = 9, **P < 0.01, ns indicates not significant. Control means treatment of negative control siRNA in A, C, D-F and means treatment of negative control lentivirus in B.

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