Fig. 4: Reversal of ADM by TSA in p48Cre/+ mouse acini.
From: Pharmacological inhibition and reversal of pancreatic acinar ductal metaplasia
p48Cre/+ mouse pancreatic acini underwent ADM over 3 days of culture (A) and were then exposed to DMSO (B) or 1 µM TSA (C) for 3 additional days followed by Calcein AM viability staining. Shown are the high content images, with the enlarged sections (dashed lines) in the lower panels. Scale bars in the top images represent 500 µm and in the lower images 100 µm. D Acini and ducts were microscopically counted from four sections of the high content images. E Expression of acinar and ductal genes from TSA treated p48Cre/+ mouse pancreatic organoids following the identical treatment as in (A–C). Data are presented as fold-change normalized to 18S rRNA and relative to the untreated control. Mean ± SD from duplicate experiments. *P < 0.05; ***P < 0.001.
