Fig. 1: Functional analysis of THP-1 and HL-60 cells with ABAT gene knockdown and HMAs treatment. | Cell Death Discovery

Fig. 1: Functional analysis of THP-1 and HL-60 cells with ABAT gene knockdown and HMAs treatment.

From: ABAT gene expression associated with the sensitivity of hypomethylating agents in myelodysplastic syndrome through CXCR4/mTOR signaling

Fig. 1

A mRNA and protein expression of ABAT gene in THP-1 and HL-60 cells after ABAT interference. B GABAT enzyme activity in both cell lines with ABAT knockdown. C Evaluation of relative cell number in ABAT silenced cells treated with HMAs. Representative figures (D) and percentage (E) of apoptosis in ABAT silenced cells treated with HMAs. F Cell cycle analysis after ABAT interference and HMAs treatment. Relative expression of CD11b and CD15 in ABAT silenced THP-1 (G) and HL-60 (H) cells with HMAs treatment. Data were represented as mean ± SEM from three biological samples. *P < 0.05, **P < 0.01. Student’s t-test (two groups) or one-way ANOVA analysis followed by Dunnett’s test (multiple groups). ns. not significant.

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