Fig. 5: CXCR4/mTOR regulation demonstrated in vitro and prognosis analysis of ABAT gene expression in vivo. | Cell Death Discovery

Fig. 5: CXCR4/mTOR regulation demonstrated in vitro and prognosis analysis of ABAT gene expression in vivo.

From: ABAT gene expression associated with the sensitivity of hypomethylating agents in myelodysplastic syndrome through CXCR4/mTOR signaling

Fig. 5

A Cell number in THP-1 and HL-60 cells treated with RAPA. B Relative expression of CXCR4 gene in cells with RAPA treatment. Cell apoptosis in ABAT silenced THP-1 (C) and HL-60 (D) cells treated with RAPA. Percentage of apoptosis in ABAT silenced THP-1 (E) and HL-60 (F) cells after RAPA and HMAs treatment. G CXCR4 overexpression in THP-1 and HL-60 cells. H Protein expression of p-mTOR, mTOR, and CXCR4 in the above two cell lines with CXCR4 overexpression. Percentage of apoptosis in cells with ABAT and CXCR4 overexpression and DAC (I) and AZA (J) treatment. K The survival of NOD/SCID mice injected with ABAT silenced THP-1 cells and HMAs treatment (log-rank test). L Schematic of ABAT/CXCR4/mTOR regulation and sensitivity to HMAs. Data were represented as mean ± SEM from three biological samples. *P < 0.05, **P < 0.01, ***P < 0.001. Student’s t-test (two groups) and one-way ANOVA analysis followed by Dunnett’s test (multiple groups). ns. not significant.

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