Fig. 1: CircPGD expression increases within GC cells and tissues. | Cell Death Discovery

Fig. 1: CircPGD expression increases within GC cells and tissues.

From: Circular RNA circPGD contributes to gastric cancer progression via the sponging miR-16-5p/ABL2 axis and encodes a novel PGD-219aa protein

Fig. 1

A Expression levels of PGD-derived circRNAs in GC tissues. B Sketch map showing circPGD forming by exonal circularization within PGD gene. According to PCR product sequencing, circPGD was spliced from the head to the tail. C Gel electrophoresis suggested that PGD existed in the canonical and back-spliced type with/without RNaseR application within gDNA and cDNA in GC cells. D and E Nuclear-cytoplasmic fractionation and RNA-FISH assay revealed the cytoplasmic and nuclear location of circPGD in MGC-803 cells, scale bars = 25 μm. F Relative circPGD level in 50 paired fresh normal gastric tissues and GC tissues. G qRT-PCR detected circPGD levels within GES-1 cells as well as three GC cell lines. *p < 0.05, **p < 0.01, ***p < 0.001.

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