Fig. 8: CircPGD encodes a novel PGD-219aa protein and promotes GC cell growth and migration while suppressing their apoptosis. | Cell Death Discovery

Fig. 8: CircPGD encodes a novel PGD-219aa protein and promotes GC cell growth and migration while suppressing their apoptosis.

From: Circular RNA circPGD contributes to gastric cancer progression via the sponging miR-16-5p/ABL2 axis and encodes a novel PGD-219aa protein

Fig. 8

A Prediction of open reading frame (ORF) containing the reversed spliced sites in circPGD. B Western blotting detected whether circPGD-Flag plasmids expressed Flag proteins in 293T cells. C Protein purification in MGC-803 cells subject to transfection with circPGD-219aa-Flag and Co-IP using Flag antibody, followed by validation through western blotting. LC–MS/MS was applied for identifying peptide sequences of collected proteins. The front (upper right) and the end (lower right) amino acid sequences are displayed. D–H The function of PGD-219aa-Flag plasmids in GC cells was monitored. D Transwell migration, E colony-formation, F CCK-8, G wound healing and H apoptosis assays result compared with the control vector in GC cells. I Sample maps of circPGD-219aa plasmids. J Western blotting detected PGD-219aa expression in GC cell lines transfected with vector, circPGD-219aa-Flag, cricPGD-219aa-mut-Flag, and PGD-219aa plasmids using the Flag antibody. K Wound healing, L CCK-8, M colony-formation, N Transwell migration, and O apoptosis assays analyzed the effects of empty vector, as well as circPGD-219aa-Flag, cricPGD-219aa-mut-Flag and PGD-219aa plasmids on GC cell migration, proliferation, and apoptosis. P, Q, and R Western blotting detected whether PGD-219aa affected EMT, as well as apoptosis- and signaling pathway-related proteins.

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