Fig. 1: ZIC5 is overexpressed in human PCa tissues and cell lines.
From: AR-regulated ZIC5 contributes to the aggressiveness of prostate cancer
A ZIC5 expression in human cancerous and normal prostate tissues in TCGA database from UCSC Xena platform. B Kaplan–Meier survival analysis based on ZIC5 expression in PCa patients. C Expression of ZIC5 in localized tumor and metastatic PCa (M-PCa) patient samples (GEO dataset GSE6919). *P < 0.05 vs. localized PCa (L-PCa) tumors. D IHC analysis of ZIC5 expression in BPH, localized PCa and adjacent normal tissue, and metastatic PCa specimens. Corresponding IHC scores were obtained according to the percentage of positive cells and the intensity of staining. N.S. (nonsignificant), P > 0.05 vs. BPH, *P < 0.05 vs. BPH, #P < 0.05 vs. localized PCa tumors. E Western blot images depicting ZIC5 expression in clinical tissues. F RT-qPCR analysis of relative ZIC5 mRNA levels in clinical tissues. N.S., P > 0.05 compared to BPH. *P < 0.05, compared to BPH. #P < 0.05, compared to L-PCa. G Analysis of relative ZIC5 expression levels in normal prostate epithelial RWPE1 cells and PCa cell lines by RT-qPCR and western blot assays. Data are presented as means ± SD (n = 3). *P < 0.05 vs. RWPE1 data.
