Fig. 5: AR directly regulates the transcription of miR-27b-3p.
From: AR-regulated ZIC5 contributes to the aggressiveness of prostate cancer
A Putative androgen response elements (AREs) on the miR-27b-3p promoter region. B ChIP analysis of AR occupancy on the promoter region of miR-27b-3p in C4-2B cells. Purified IgG was used as control. *P < 0.05 vs. IgG. PSA were used as positive controls. C ChIP-qPCR analysis to assess specific binding of SRC-1, SRC-2, SRC-3, and AR to the miR-27b-3p promoter in C4-2B cells transfected with control or AR-targeted siRNA. Purified IgG was used as control. *P < 0.05 vs. NC, N.S. P > 0.05 vs. NC. D ChIP-qPCR analysis to assess specific binding of SRC-1, SRC-2, SRC-3, and AR to the miR-27b-3p promoter in 22RV1 cells transfected with control or SRC-3-targeted siRNA. Purified IgG was used as control. *P < 0.05 vs. NC, N.S. P > 0.05 vs. NC. E Luciferase-based detection of miR-27b-3p promoter activity in C4-2B cells exposed to the indicated treatments. *P < 0.05 vs. NC, #P < 0.05 vs. DHT, N.S. P > 0.05 vs. NC or DHT. F RT-qPCR analysis of relative miR-27b-3p levels in C4-2B cells treated with or without DHT following administration of bufalin (50 nM, 24 h) or indicated SRC-targeted siRNA (48 h). *P < 0.05 vs. NC, #P < 0.05 vs. DHT, N.S. P > 0.05 vs. NC or DHT. G ChIP-qPCR analysis to assess specific binding of H3K9Ac and H3K9Me2 to the miR-27b-3p promoter in C4-2B cells transfected with control or AR-targeted siRNA. Purified IgG was used as control. *P < 0.05 vs. NC. H RT-qPCR analysis of relative miR-27b-3p levels in C4-2B and 22RV1 cells treated with or without DHT following administration of VST (1 μM, 24 h) or bufalin (50 nM, 24 h) or SRC3-targeted siRNA (48 h). *P < 0.05 vs. NC, #P < 0.05 vs. DHT, &P < 0.05 vs. DHT + VST.
